Abstract
Bovine herpesvirus 4 (BoHV-4) is a gamma-herpesvirus with no clear disease association. A recombinant BoHV-4 (BoHV-4EGFPΔTK) expressing Green Fluorescent Protein (EGFP), was successfully used to infect F98 rat glioma cells. BoHV-4EGFPΔTK was injected into the lateral ventricle of the rat brain. Histology and immunohistochemistry showed that ependymal and rostral migratory stream cells were transduced while neurons were not. Clinical scores, evaluated for 90 days, indicated that the virus was non neuropathogenic, suggesting this virus is a suitable vector for brain tumor gene therapy.
Highlights
Bovine herpesvirus 4 (BoHV-4) is a gamma-herpesvirus with no clear disease association [1], suggesting it as a suitable vector for gene therapy
We have previously demonstrated that BoHV-4 does not replicate in the mouse brain and that infection was restricted to ependymal and rostral migratory stream (RMS) regions after viral injection in the lateral ventricle of the mouse brain [10]
The infection and transduction of rat glioma cells in vitro was explored, employing the rat glioma F98 cell line, which were maintained in growth medium
Summary
Bovine herpesvirus 4 (BoHV-4) is a gamma-herpesvirus with no clear disease association [1], suggesting it as a suitable vector for gene therapy. BoHV-4 has been isolated from different tissues and has been show to establish a persistent infection in its natural host, the cattle, and in the experimental animal, the rabbit [2,3]. BoHV-4 does replicate and cause a cytopathic effect in a number of immortalized cell lines and primary cell cultures [8,9].
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