Abstract
BackgroundATP-binding cassette (ABC) protein family encode for membrane proteins involved in the transport of various biomolecules through the cellular membrane. These proteins have been identified in all taxa and present important physiological functions, including the process of insecticide detoxification in arthropods. For that reason the ectoparasite Caligus rogercresseyi represents a model species for understanding the molecular underpinnings involved in insecticide drug resistance.Methodsllumina sequencing was performed using sea lice exposed to 2 and 3 ppb of deltamethrin and azamethiphos. Contigs obtained from de novo assembly were annotated by Blastx. RNA-Seq analysis was performed and validated by qPCR analysis.ResultsFrom the transcriptome database of C. rogercresseyi, 57 putative members of ABC protein sequences were identified and phylogenetically classified into the eight subfamilies described for ABC transporters in arthropods. Transcriptomic profiles for ABC proteins subfamilies were evaluated throughout C. rogercresseyi development. Moreover, RNA-Seq analysis was performed for adult male and female salmon lice exposed to the delousing drugs azamethiphos and deltamethrin. High transcript levels of the ABCB and ABCC subfamilies were evidenced. Furthermore, SNPs mining was carried out for the ABC proteins sequences, revealing pivotal genomic information.ConclusionsThe present study gives a comprehensive transcriptome analysis of ABC proteins from C. rogercresseyi, providing relevant information about transporter roles during ontogeny and in relation to delousing drug responses in salmon lice. This genomic information represents a valuable tool for pest management in the Chilean salmon aquaculture industry.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-015-0801-x) contains supplementary material, which is available to authorized users.
Highlights
ATP-binding cassette (ABC) protein family encode for membrane proteins involved in the transport of various biomolecules through the cellular membrane
Identification of ABC transporter subfamily genes from C. rogercresseyi Through BLASTx analysis, 57 full or partial sequences were identified ABC transporter from transcriptome database described by Gallardo-Escárate et al [35] and by using arthropod ABC transporter sequences described in public databases as a reference (Additional file 1: Table S2)
Phylogenetic analysis revealed a relationship between seven C. rogercresseyi contigs and the ABCA subfamily described in D. pulex (Figure 1)
Summary
ATP-binding cassette (ABC) protein family encode for membrane proteins involved in the transport of various biomolecules through the cellular membrane These proteins have been identified in all taxa and present important physiological functions, including the process of insecticide detoxification in arthropods. A sea louse, is a widely prevalent parasite in the Chilean aquaculture industry [1,2]. This ectoparasite belongs to the Caligidae family, which includes species such as Lernaeocera branchialis, Caligus clemensi, and Lepeophtheirus salmonis, with this final species having greater prevalence in countries such as Scotland, Norway, Canada, and England [3]. One of the protein families which has shown detoxifying effects against drugs are the ABC transporters.
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