Transcriptomic Analysis of Xylan Oligosaccharide Utilization Systems in Pediococcus acidilactici Strain BCC-1.

Abstract

Xylan oligosaccharides (XOS) are the hydrolysates of xylan. To compare the proliferation effect of XOS, glucose, fructo oligosaccharides (FOS), xylose, XOS, and a media without carbohydrate source (control) on Pediococcus acidilactici strain BCC-1, the de novo sequencing of Pediococcus acidilactici strain BCC-1 was conducted, and the underlying mechanism of prebiotic xylo oligosaccharide between xylose and XOS was revealed through transcriptomic analysis. Compared to FOS, glucose, and xylose, XOS exhibits a good performance in promoting the fermentation of Pediococcus acidilactici BCC-1. The genome of Pediococcus acidilactici BCC-1 revealed genes encoding XOS transportation and utilized related enzymes, including ATP-binding cassette (ABC) transporters, arabinofuranosidase, xylanase, xylosidase, xylose isomerase, and xylulose kinase. Transcriptome analysis showed that XOS treatment enhanced genes involving carbohydrate metabolism, an ABC transporter sugar system, pentose and glucuronate interconversions, pyruvate metabolism, and the TCA process when compared to xylose treatment. It suggested XOS treatment enhanced sugar absorption and utilization. These results are useful in the understanding of the metabolic pathway of XOS in Pediococcus acidilactici BBC-1 and may contribute to the optimization of the probiotic effect of Pediococcus acidilactici BCC-1 as novel complex feed additives.