Comparative transcriptome analysis of ATP-binding cassette (ABC) transporter genes in eri-silkworm, Samia cynthia ricini in response to 1-deoxynojirimycin

Abstract

1-Deoxynojirimycin (DNJ) as a kind of alkaloid has been confirmed that could modulate glycometabolism and has toxicity for the eri-silkworm in our previous research. On the contrary, what is the potentially defensive mechanism when the DNJ enters the eri-silkworm. Based on comparative transcriptome sequencing, we found that ATP-binding cassette (ABC) transporter genes could be induced significantly by DNJ. In this study, a total of 16 putative ABC transporter genes were identified, which can be classified into seven subfamilies, namely one ABCA, four ABCBs, three ABCCs, two ABCDs, one ABCE, three ABCFs, and two ABCGs. Phylogenetic analysis revealed that ScABCs had strong conservation with Bombyx mori. Reverse transcription quantitative PCR (RT-qPCR) suggested that 6 ABC transporters had a strong positive correlation between RT-qPCR and transcriptome data. Additionally, S. c. ricini ABC transporter C-subfamily 4 (ScABCC4), S. c. ricini ABC transporter G-subfamily 4 (ScABCG4), S. c. ricini ABC transporter A-subfamily 3 (ScABCA3) and S. c. ricini ABC transporter C-subfamily 10 (ScABCC10) showed different expression pattern in two feed dose (1% and 2% DNJ) and three time points (6h, 12h, 48h). This study provides the first study on identification, characterization and expression patterns of ABC transporter genes in S. c. ricini response to DNJ, and lays a foundation for further understanding of their physiological roles response to the alkaloid.