Abstract
An aquatic gastropod belonging to the family Neritidae, Clithon retropictus is listed as an endangered class II species in South Korea. The lack of information on its genomic background limits the ability to obtain functional data resources and inhibits informed conservation planning for this species. In the present study, the transcriptomic sequencing and de novo assembly of C. retropictus generated a total of 241,696,750 high-quality reads. These assembled to 282,838 unigenes with mean and N50 lengths of 736.9 and 1201 base pairs, respectively. Of these, 125,616 unigenes were subjected to annotation analysis with known proteins in Protostome DB, COG, GO, and KEGG protein databases (BLASTX; E ≤ 0.00001) and with known nucleotides in the Unigene database (BLASTN; E ≤ 0.00001). The GO analysis indicated that cellular process, cell, and catalytic activity are the predominant GO terms in the biological process, cellular component, and molecular function categories, respectively. In addition, 2093 unigenes were distributed in 107 different KEGG pathways. Furthermore, 49,280 simple sequence repeats were identified in the unigenes (>1 kilobase sequences). This is the first report on the identification of transcriptomic and microsatellite resources for C. retropictus, which opens up the possibility of exploring traits related to the adaptation and acclimatization of this species.
Highlights
Mollusca comprises a highly diversified group of animals, with members distributed in almost every ecosystem
The ultimate goal of this study is to describe the species transcriptome and provide genetic information for future studies that aim at understanding phenotypes, adaptation, inbreeding, and conservation priorities
The raw reads were subjected to quality control, which included trimming of adapter sequences using the Cutadapt program
Summary
Mollusca comprises a highly diversified group of animals, with members distributed in almost every ecosystem. The molluscan habitats include terrestrial mountaintops and the hot vents and cold seeps of the deep sea. These creatures have adapted to terrestrial, marine, and freshwater habitats and constitute the second most speciose phylum of all animals. Gastropods have successfully occupied all marine and freshwater habitats and are the only group of molluscs to have invaded the land They have been the subject of paleobiological and numerous other ecological, evolutionary, physiological, and behavioural studies [1,2]. Their undisputed variety of morphology, structure, and habits, augmented by their utility to humans throughout history as a source of food, ornaments, tools, and even pets, has made the members of the gastropod community valuable for research investigations [3,4]
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