Transcriptomic Analysis of Staphylococcus epidermidis Biofilm-Released Cells upon Interaction with Human Blood Circulating Immune Cells and Soluble Factors.

Angela França,Manuel Vilanova,Nuno Cerca,Gerald B Pier

doi:10.3389/fmicb.2016.01143

Abstract

This study was funded by the Portuguese Foundation for Science and Technology (FCT) by the project with the reference FCOMP-01-012014-FEDER-041246 (EXPL/BIA-MIC/0101/2013), the strategic funding of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI-01-0145-FEDER-006684), BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the. scope of Norte2020 - Programa Operational Regional do Norte. NC is an Investigador FCT. AT is supported by the FCT fellowship SFRH/BPD/99961/2014. The hinders had no role in study design, data collection and interpretation, or decision to submit the work for publication.

Highlights

The colonization of indwelling medical devices by biofilm-forming bacteria is one of the major causes of healthcare-associated infections (Percival et al, 2015)
S. epidermidis biofilms are classically associated with the development of chronic infections (Costerton et al, 1999), the release of cells from the biofilm has been associated with onset of acute infections such as embolic events of endocarditis (Pitz et al, 2011), bacteremia, or even septicemia (Cole et al, 2016)
Since bloodstream infections are one of the most frequent complications caused by S. epidermidis biofilm disassembly (Cole et al, 2016), a comprehensive analysis of the interplay between S. epidermidis biofilm-released cells (BRC) and hosts’ blood components would be invaluable

Summary

Introduction

The colonization of indwelling medical devices by biofilm-forming bacteria is one of the major causes of healthcare-associated infections (Percival et al, 2015). Staphylococcus epidermidis, a biofilm-forming commensal bacterium that inhabits human skin and mucosae, is considered one of most important causes of medical devices-related infections, being associated with the use of intravascular catheters (Mack et al, 2013). Since bloodstream infections are one of the most frequent complications caused by S. epidermidis biofilm disassembly (Cole et al, 2016), a comprehensive analysis of the interplay between S. epidermidis biofilm-released cells (BRC) and hosts’ blood components would be invaluable. As the first step toward the understanding of this interaction, we have characterized, using RNA sequencing (RNAseq) technology, the transcriptome of S. epidermidis BRC upon interaction with whole human blood, polymorphonuclear, or mononuclear leukocytes and plasma

Objectives

Methods

Conclusion