Transcriptomic Analysis of Leaf in Tree Peony Reveals Differentially Expressed Pigments Genes.

Jianrang Luo,Lixin Niu,Qianqian Shi,Yanlong Zhang

doi:10.3390/molecules22020324

Jianrang Luo, Lixin Niu + Show 2 more

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https://doi.org/10.3390/molecules22020324

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Abstract

Tree peony (Paeonia suffruticosa Andrews) is an important traditional flower in China. Besides its beautiful flower, the leaf of tree peony has also good ornamental value owing to its leaf color change in spring. So far, the molecular mechanism of leaf color change in tree peony is unclear. In this study, the pigment level and transcriptome of three different color stages of tree peony leaf were analyzed. The purplish red leaf was rich in anthocyanin, while yellowish green leaf was rich in chlorophyll and carotenoid. Transcriptome analysis revealed that 4302 differentially expressed genes (DEGs) were upregulated, and 4225 were downregulated in the purplish red leaf vs. yellowish green leaf. Among these DEGs, eight genes were predicted to participate in anthocyanin biosynthesis, eight genes were predicted involved in porphyrin and chlorophyll metabolism, and 10 genes were predicted to participate in carotenoid metabolism. In addition, 27 MYBs, 20 bHLHs, 36 WD40 genes were also identified from DEGs. Anthocyanidin synthase (ANS) is the key gene that controls the anthocyanin level in tree peony leaf. Protochlorophyllide oxido-reductase (POR) is the key gene which regulated the chlorophyll content in tree peony leaf.

Highlights

Tree peony (Paeonia suffruticosa Andr.) is a woody shrub of the genus Paeonia and familyPaeoniaceae, and is a traditional ornamental flower because of its large, showy, and colorful flowers [1].Besides its beautiful flower, the leaf of tree peony has good ornamental value owing to its leaf color change in spring.Coloration of plant organs such as flowers, fruit and leaves is governed by pigments in plants [2].There are four main classes of pigments for coloration in plants: flavonoids/anthocyanins, chlorophylls, carotenoids, and betalains [3]
The leaf of tree peony has good ornamental value owing to its leaf color change in spring
The anthocyanidins level of the purplish red leaf was significantly higher than that of yellowish green leaf, while chlorophylls and carotenoid level of purplish red leaf was significantly lower than that of the yellowish green leaf. These results indicated that purplish red leaf in tree peony could be attributed to anthocyanidin accumulation

Summary

Introduction

Tree peony (Paeonia suffruticosa Andr.) is a woody shrub of the genus Paeonia and familyPaeoniaceae, and is a traditional ornamental flower because of its large, showy, and colorful flowers [1].Besides its beautiful flower, the leaf of tree peony has good ornamental value owing to its leaf color change in spring.Coloration of plant organs such as flowers, fruit and leaves is governed by pigments in plants [2].There are four main classes of pigments for coloration in plants: flavonoids/anthocyanins, chlorophylls, carotenoids, and betalains [3]. Paeoniaceae, and is a traditional ornamental flower because of its large, showy, and colorful flowers [1]. The leaf of tree peony has good ornamental value owing to its leaf color change in spring. Coloration of plant organs such as flowers, fruit and leaves is governed by pigments in plants [2]. There are four main classes of pigments for coloration in plants: flavonoids/anthocyanins, chlorophylls, carotenoids, and betalains [3]. Flavonoids, anthocyanins, acting as major pigments that responsible for the orange to blue colors found in many plant flowers, leaves, fruits, seeds, and other tissues [2,3,4]. Carotenoid belong to the isoprenoid pigments, which are usually responsible for yellow-orange colors [6,7]

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