Abstract
Lignocellulosic biomass (LCB) is a promising feedstock for producing value-added chemicals, with Lactiplantibacillus plantarum emerging as a potential cell factory due to its ability to metabolize lignocellulosic sugars. However, LCB preprocessing generates toxic compounds, such as furfural and 5-hydroxymethylfurfural (HMF), which inhibit microbial growth. In this study, we explored the transcriptional responses of L. plantarum JGR2 to furfural, HMF, and their combination using RNA-seq analysis. Notably, the pyr operon, involved in pyrimidine metabolism, was downregulated across all treatments, while 1,3-propanediol dehydrogenase (dhaT) was upregulated under furfural exposure. Recombinant E. coli overexpressing DhaT successfully converted furfural into the less toxic furfuryl alcohol. The identification of DhaT as a furfural reductase, along with the transcriptomic data on L. plantarum's response to furan aldehydes, can guide the development of LCB-driven L. plantarum as a novel microbial cell factory.
Published Version
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