Abstract
BackgroundThe newly discovered reversible N6-methyladenosine (m6A) modification plays an important regulatory role in gene expression. Long non-coding RNAs (lncRNAs) participate in Marek’s disease virus (MDV) replication but how m6A modifications in lncRNAs are affected during MDV infection is currently unknown. Herein, we profiled the transcriptome-wide m6A modification in lncRNAs in MDV-infected chicken embryo fibroblast (CEF) cells.ResultsMethylated RNA immunoprecipitation sequencing results revealed that the lncRNA m6A modification is highly conserved with MDV infection increasing the expression of lncRNA m6A modified sites compared to uninfected cell controls. Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that lncRNA m6A modifications were highly associated with signaling pathways associated with MDV infection.ConclusionsIn this study, the alterations seen in transcriptome-wide m6A occurring in lncRNAs following MDV-infection suggest this process plays important regulatory roles during MDV replication. We report for the first time profiling of the alterations in transcriptome-wide m6A modification in lncRNAs of MDV-infected CEF cells.
Highlights
The newly discovered reversible N6-methyladenosine (m6A) modification plays an important regulatory role in gene expression
Transcriptome-wide m6A modifications in Long non-coding RNAs (lncRNAs) after Md5 infection RNA-sequencing and transcriptome analyses were performed on mock control and Md5-infected chicken embryo fibroblast (CEF) cells following successful construction of cDNA libraries (Fig. 1)
277 m6A peaks and 228 m6A modified genes were detected in both the Md5infected and control groups. These results indicated that the incidence of the m6A modification in lncRNAs was higher in the Md5 infected group compared to the control group
Summary
The newly discovered reversible N6-methyladenosine (m6A) modification plays an important regulatory role in gene expression. Long non-coding RNAs (lncRNAs) participate in Marek’s disease virus (MDV) replication but how m6A modifications in lncRNAs are affected during MDV infection is currently unknown. Non-coding RNAs (ncRNAs) constitute a varied group of RNA molecules that do not encode functional proteins. Post-transcriptional RNA modifications of lncRNAs may change the expression and activity of mRNAs, ncRNAs and proteins, resulting in epigenetic changes in infected cells. Expression profiling of long intergenic non-coding RNA (lincRNAs) has been previously reported in the chicken bursa following MDV infection. Other comprehensive work reporting lncRNA expression profiling indicated that five lncRNAs were strongly related to the expression of MDV and host protein coding genes, and these lncRNAs may play significant roles during MDV-induced tumorigenesis [10]. Whether and how lncRNA expression is regulated during MDV replication is unclear
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