Transcriptome sequencing of the central nervous system to identify the neuropeptides and neuropeptide receptors of Antheraea pernyi

Abstract

Neuropeptides and neuropeptide receptors are crucial regulators for the behavior, lifecycle, and physiology of insects and are mainly produced and released from the neurosecretory cells of the central nervous system (CNS). In this study, RNA-seq was employed to investigate the transcriptome profile of the CNS which is composed of the brain and ventral nerve cord (VNC) of Antheraea pernyi. From the data sets, a total of 18 and 42 genes were identified, which respectively encode the neuropeptides and neuropeptide receptors involved in regulating multiple behaviors including feeding, reproductive behavior, circadian locomotor, sleep, and stress response and physiological processes such as nutrient absorption, immunity, ecdysis, diapause, and excretion. Comparison of the patterns of expression of those genes between the brain and VNC showed that most had higher levels of expression in the brain than VNC. Besides, 2760 differently expressed genes (DEGs) (1362 up-regulated and 1398 down-regulated ones between the B and VNC group) were also screened and further analyzed via gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses. The results of this study could provide comprehensive profiles of the neuropeptides and neuropeptide receptors of A. pernyi CNS and lay the foundation for further research into their functions.