Abstract
This study describes the muscle transcriptome profile of Bandur breed, a consumer favoured, meat type sheep of India. The transcriptome was compared to the less desirable, unregistered local sheep population, in order to understand the molecular factors related to muscle traits in Indian sheep breeds. Bandur sheep have tender muscles and higher backfat thickness than local sheep. The longissimus thoracis transcriptome profiles of Bandur and local sheep were obtained using RNA sequencing (RNA Seq). The animals were male, non-castrated, with uniform age and reared under similar environment, as well as management conditions. We could identify 568 significantly up-regulated and 538 significantly down-regulated genes in Bandur sheep (p≤0.05). Among these, 181 up-regulated and 142 down-regulated genes in Bandur sheep, with a fold change ≥1.5, were considered for further analysis. Significant Gene Ontology terms for the up-regulated dataset in Bandur sheep included transporter activity, substrate specific transmembrane, lipid and fatty acid binding. The down-regulated activities in Bandur sheep were mainly related to RNA degradation, regulation of ERK1 and ERK2 cascades and innate immune response. The MAPK signaling pathway, Adipocytokine signaling pathway and PPAR signaling pathway were enriched for Bandur sheep. The highly connected genes identified by network analysis were CNOT2, CNOT6, HSPB1, HSPA6, MAP3K14 and PPARD, which may be important regulators of energy metabolism, cellular stress and fatty acid metabolism in the skeletal muscles. These key genes affect the CCR4-NOT complex, PPAR and MAPK signaling pathways. The highly connected genes identified in this study, form interesting candidates for further research on muscle traits in Bandur sheep.
Highlights
India possesses 6% of the world’s sheep population [1], with 42 registered breeds & several lesser known ones [2]
The body biometry and some carcass traits of the animals that were used for transcriptome analysis by RNA sequencing were recorded
The fatty acid and amino acid profile revealed that Bandur sheep had a significantly higher level of oleic acid and histidine (p 0.05) respectively (S4 and S5 Table)
Summary
India possesses 6% of the world’s sheep population [1], with 42 registered breeds & several lesser known ones [2]. Bandur is a famous mutton type sheep breed of India which is preferred by consumers for its palatability. It fetches a higher price than mutton from other breeds in the same area [3]. The Bandur animals have a compact body, white coat and a typical reversed U-shaped conformation from the rear [4] Another population of sheep found in the same area, which is not registered is referred as the local sheep.The local sheep are medium built, heavier than Bandur, with a light brown coat colour. Despite the local popularity and market potential, no scientific information is available on the uniqueness of its meat quality or muscle traits. Some information is available on the carcass traits for Bandur sheep [5,6,7], but genetic analysis is still lacking
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