Transcriptome Comparison of Defense Responses in the Rice Variety 'Jao Hom Nin' Regarding Two Blast Resistant Genes, Pish and Pik.

Athipat Ngernmuen,Worrawit Suktrakul,Chatchawan Jantasuriyarat,Sureeporn Kate-Ngam

doi:10.3390/plants9060694

Athipat Ngernmuen, Worrawit Suktrakul + Show 2 more

Open Access

https://doi.org/10.3390/plants9060694

Copy DOI

Abstract

Jao Hom Nin (JHN) is a Thai rice variety with broad-spectrum resistant against rice blast fungus. JHN contains two rice blast resistant genes, Pish and Pik, located on chromosome 1 and on chromosome 11, respectively. To understand the blast resistance in JHN, the study of the defense mechanism related to the Pish and Pik genes is crucial. This study aimed to dissect defense response genes between the Pish and Pik genes using the RNA-seq technique. Differentially expressed genes (DEGs) of Pish and Pik backcross inbred lines were identified between 0 and 24 h after inoculation with rice blast spore suspension. The results showed that 1248 and 858 DEGs were unique to the Pish and Pik lines, respectively. The wall-associated kinase gene was unique to the Pish line and the zinc-finger-containing protein gene was unique to the Pik line. Pathogenicity-related proteins PR-4 and PR-10 were commonly found in both Pish and Pik lines. Moreover, DEGs functionally categorized in brassinosteriod, jasmonic acid, and salicylic acid pathways were detected in both Pish and Pik lines. These unique and shared genes in the Pish and Pik rice blast defense responses will help to dissect the mechanisms of plant defense and facilitate rice blast breeding programs.

Highlights

Rice, Oryza sativa L., is an important cereal crop and serves as a major source of energy for more than half of the global population
The development of RD6 backcross inbred lines introgressed by the rice-blast-resistant QTLs from Jao Hom Nin (JHN) cultivar by marker-assisted selection was conducted at the Rice Science Center and Rice Gene Discovery Unit, Kasetsart University, Kamphaengsaen campus
Ninety-four polymorphic DNA markers between JHN and RD6 were used to construct the graphical genotypes of BC4 F2 rice-blast-resistant Q1 and Q11 backcross inbred lines (BILs)

Summary

Introduction

Oryza sativa L., is an important cereal crop and serves as a major source of energy for more than half of the global population. Rice blast disease, caused by an ascomycete fungus Magnaporthe oryzae, is one of the most destructive diseases in rice production worldwide. All above-ground rice plants can be infected by this fungus. This disease frequently occurs in temperate rice growing regions. An outbreak of the rice blast disease results in rice yield loss. The rice blast disease is responsible for 30% global rice production losses and results in an increase of costs to control this disease [4,5]. One of the potential methods to control this disease is using rice-blast-resistant cultivars [6]. The resistance capability of the resistant cultivars is explained by Plants 2020, 9, 694; doi:10.3390/plants9060694 www.mdpi.com/journal/plants

Objectives

Methods

Findings

Discussion

Conclusion