Development and Application of &lt;i&gt;Pi-k&lt;sup&gt;h&lt;/sup&gt;&lt;/i&gt; Co-dominant Marker for Rice Blast Resistance Gene

Shifei Sang,Yanan Wang,Shengdong Ji,Wenling Zhang,Junyi Wang,Mengyu Cao,Jianqiang Zhang,Jing Zhou

doi:10.5376/mpb.2021.12.0004

Abstract

Rice, as one of the staple food crops in the world, is reduced by 10~30% every year due to the damage of rice blast. However, the application of marker assistant selection (MAS) with rice disease resistance genes can be effectively, accurately and rapidly used to breed rice blast resistant varieties. The rice blast resistance gene  Pi-k h  has been proved to have broad-spectrum resistance to the rice blast. Therefore, the selection and utilization of  Pi-k h  gene by MAS in rice disease resistance breeding possess high application value for improving rice blast resistance. In this study, based on two InDel differences in Coding sequence (CDS) region of  Pi-k h  gene, a co-dominant functional molecular marker was developed at the conservative position of the sequence, which was named ZJPikh. The marker was used in the disease resistance breeding with rice blast susceptible variety “Yudao 518” as female parent and rice blast resistant variety “Shida 68” as male parent. The genotype of gene  Pi-k h  related with rice blast resistance in F2 isolated population and high generation plants were verified by pedigree method and MAS. A new rice blast resistant third-grade variety “Yudao 58” with  Pi-k h  was successfully bred. At the same time, the  Pi-k h  genotypes of 21 main rice varieties were identified, which provided technical support for the targeted and efficient breeding of blast resistant varieties carrying  Pi-k h  gene through MAS technology. Our research provides convenience for identifying rice blast resistance germplasm resources and molecular marker-assisted disease resistance breeding.

Highlights

Rice is one of the main food crops in the world, the world’s arable land area is declining, and the population is increasing
At least 35 rice blast resistance genes have been cloned in rice (Wang et al, 2017a; Zhao et al, 2018), of which the rice blast resistance genes have been successfully utilized through marker assistant selection (MAS) technology and new blast-resistant lines or varieties have been selected, such as applying the KASP marker developed based on Pi9, and quickly screened homozygous lines containing Pi9 resistance genes through MAS (Wei et al, 2019); The developed Pigm marker was used to identify disease-resistant plants in the F2 population of Huaidao 9/Gumei 4, Molecular Plant Breeding 2021, Vol.12, No.4, 1-9 http://genbreedpublisher.com/index.php/mpb and high-resistant rice blast lines were obtained (Chen et al, 2020)
In this study, based on the two InDel differences in the coding sequence (CDS) region of the Pi-kh gene, a co-dominant functional molecular marker was developed at a conservative position in the sequence, and the marker was applied to the breeding of new resistant lines or varieties with "Yudao 518" as female parent and "Shida 68" as male parent by molecular marker assistant select (MAS)

Summary

Introduction

Rice is one of the main food crops in the world, the world’s arable land area is declining, and the population is increasing. Rice blast is known as rice fever, commonly known as fire blast, neck blast, hanging head blast, rice cancer, etc It is one of the most serious fungal diseases that affect the safety of rice production (Zhao et al, 2019). At least 35 rice blast resistance genes have been cloned in rice (Wang et al, 2017a; Zhao et al, 2018), of which the rice blast resistance genes have been successfully utilized through MAS technology and new blast-resistant lines or varieties have been selected, such as applying the KASP marker developed based on Pi9, and quickly screened homozygous lines containing Pi9 resistance genes through MAS (Wei et al, 2019); The developed Pigm marker was used to identify disease-resistant plants in the F2 population of Huaidao 9/Gumei 4, Molecular Plant Breeding 2021, Vol., No.4, 1-9 http://genbreedpublisher.com/index.php/mpb and high-resistant rice blast lines were obtained (Chen et al, 2020). The Pi-kh gene is selected by MAS and used in rice disease resistance breeding, which has high application value for improving rice blast resistance. This study provided technical support for the cultivation of Pi-kh gene resistant rice varieties by molecular marker assisted selection

Methods

Results

Conclusion