Abstract
Background337S is a novel bi-pole-photo-thermo-sensitive genic male sterile line in wheat, and sensitive to both long day length/high temperature and short day length/low temperature condition. Although the regulatory function of MicroRNAs (miRNAs) in reproductive development has been increasingly studied, their roles in pre-meiotic and meiotic cells formation of plants have not been clearly explored. Here, we explored the roles of miRNAs in regulating male sterility of 337S at short day length/low temperature condition.ResultsSmall RNA sequencing and degradome analyses were employed to identify miRNAs and their targets in the 337S whose meiotic cells collapsed rapidly during male meiotic prophase, resulting in failure of meiosis at SL condition. A total of 102 unique miRNAs were detected. Noticeably, the largest miRNA family was MiR1122. The target CCR4-associated factor 1 (CAF1) of miR2275, a subunit of the Carbon Catabolite Repressed 4-Negative on TATA-less (CCR4-NOT) complex, contributes to the process of early meiosis, and was first identified here. Further studies showed that the expression of several pivotal anther-related miRNAs was altered in 337S at SL condition, especially tae-miR1127a, which may be related to male sterility of 337S. Here, we also identified a new member of SWI/SNF factors SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A, member 3-like 3 (SMARCA3L3) targeted by tae-miR1127a, whose function might be involved in faithful progression of meiosis in male reproductive cells.ConclusionThe miRNA-target interactions of tae-miR2275-CAF1 and tae-miR1127a-SMARCA3L3 might be involved in regulating male fertility in 337S. Our results also implied that multiple roles for SMARCA3L3 and CAF1 in DNA repair and transcriptional regulation jointly orchestrated a tight and orderly system for maintaining chromatin and genome integrity during meiosis.
Highlights
337S is a novel bi-pole-photo-thermo-sensitive genic male sterile line in wheat, and sensitive to both long day length/high temperature and short day length/low temperature condition
The growth of anthers prior to the microspore stage was divided into 3 phases: early anther development stage, microspore mother cell stage and meiosis stage according to the length of the wheat spikes planted in the field
337S is a novel BP-PhotoperiodThermo-Sensitive Genic Male Sterile (PTGMS) line in wheat, sensitive to both long day length/high temperature and short day length/low temperature condition. miRNAs are involved in reproductive development for many plants. taemiR2275-3p and miRNA families of MiR1120 and MiR1122 were found to be involved in the regulation of meiosis process and early anther development in wheat
Summary
337S is a novel bi-pole-photo-thermo-sensitive genic male sterile line in wheat, and sensitive to both long day length/high temperature and short day length/low temperature condition. The regulatory function of MicroRNAs (miRNAs) in reproductive development has been increasingly studied, their roles in pre-meiotic and meiotic cells formation of plants have not been clearly explored. There are two well-known male sterility systems that have been developed for hybrid seed production: Cytoplasmic Male Sterile (CMS) and PhotoperiodThermo-Sensitive Genic Male Sterile (PTGMS) [1]. Meiotic recombination is one of the most important events during the early stage of meiosis, which is initiated by the generation of DNA double strand breaks (DSBs) [5]. The CAF1 encoding a conserved subunit of the CCR4NOT complex is vital to meiotic progression, loss function of which resulted in sterility by blocking germ cell development at the pachytene stage of meiosis I in C. elegans [10]
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