Abstract
Abalone (Haliotis discus hannai) is one of the most valuable marine aquatic species in Korea, Japan and China. Tremendous exposure to bacterial infection is common in aquaculture environment, especially by Vibrio sp. infections. It’s therefore necessary and urgent to understand the mechanism of H. discus hannai host defense against Vibrio parahemolyticus infection. However studies on its immune system are hindered by the lack of genomic resources. In the present study, we sequenced the transcriptome of control and bacterial challenged H. discus hannai tissues. Totally, 138 MB of reference transcriptome were obtained from de novo assembly of 34 GB clean bases from ten different libraries and annotated with the biological terms (GO and KEGG). A total of 10,575 transcripts exhibiting the differentially expression at least one pair of comparison and the functional annotations highlight genes related to immune response, cell adhesion, immune regulators, redox molecules and mitochondrial coding genes. Mostly, these groups of genes were dominated in hemocytes compared to other tissues. This work is a prerequisite for the identification of those physiological traits controlling H. discus hannai ability to survive against Vibrio infection.
Highlights
Abalones (Gastropoda; Haliotidae) has become an increasingly important fishery and aquaculture resource with high nutrition value and widely distributed throughout the southern coasts of Korea, Japan, China and Taiwan
Abalones were infected with the intracellular injection 100 μL of V. parahemolyticus (1.2×108 colony-forming units (CFU)/mL) suspended in phosphate-buffered saline (PBS) buffer, and a control ones were injected with PBS buffer
To obtain more detailed information of H. discus hannai transcriptome, adult abalones (6-8cm shell length) were collected and cDNA was transcribed from total RNA followed by cDNA library sequencing with Illumina HiSeqTM 2000 system to obtain as many transcripts as possible
Summary
Abalones (Gastropoda; Haliotidae) has become an increasingly important fishery and aquaculture resource with high nutrition value and widely distributed throughout the southern coasts of Korea, Japan, China and Taiwan. Abalones have sedentary lifestyle along shallow rocky coast lines; these gastropods are vulnerable to capture [1]. 111,000 metric tons (mt) of abalone (from all sources) were supplied globally in 2013 with over 103,000 mt harvested directly from aquaculture facilities (http://www.fao.org/statistics/en/). China itself account for over 79% of the global abalone aquaculture product followed by South Korea. RNA-Seq Analysis of Haliotis discus hannai publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section
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