TRANSCRIPTOME ANALYSIS OF THE SPLEEN OF THE BAIKAL CISCO (LAKE BAIKAL, EASTERN SIBERIA)

Abstract

For the first time, new data have been presented on the spleen transcriptome of the Baikal cisco Coregonus migratorius Georgi, 1775 (Salmoniformes: Coregonidae), infected with parasites of different systematic groups. Transcriptomic libraries were sequenced on an Illumina NextSeq550 sequencer using the NextSeq® 550 High Output Kit v2. The de-novo transcriptome was assembled. Conserved domains and their associated Gene Ontology annotations were predicted with Blast2Go. The annotation results of the obtained transcripts found that transcripts were distributed in the spleen into the following categories: molecular functions, biological processes, and cellular components. Among the molecular functions, transcripts of enzyme binding (25.8%), transferase activity (24.7%), hydrolase activity (24.4%), catalytic activity affecting proteins (22.2%), and DNA binding (21%) predominated. Biological processes were dominated by transcripts of cellular processes (46.7%), metabolic processes (38.6%), biological regulation (38.2%), and regulation components of biological processes (36.6%). The category of cellular GO components identified terms, namely cytoplasmic vesicles (25.9%), cytoplasmic membranes (23.1%), nucleoplasm (22.1%), cytoskeletal part (18.6%), cytosol and cellular compounds (17%) in a greater extent.