Transcriptome analysis of hepatic injury caused by delayed resuscitation following severe burns in rats.

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Delayed resuscitation (DR) can induce hepatic reperfusion injury after severe burns. The underlying molecular mechanisms of DR-induced hepatic injury remain unidentified. This study sought to predict candidate genes and molecular pathways in a DR-induced hepatic injury preclinical model. Rats were randomized into three groups: the sham group, the DR group, which had third-degree burns covering 30% of the body surface area and delayed resuscitation, and the ER group, in which early resuscitation was administered. The liver tissue was harvested for the purpose of evaluating hepatic injury and performing transcriptome sequencing. Differentially expressed genes (DEGs) for DR versus Sham and ER versus DR were analyzed respectively. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes and Ingenuity pathway analyses were performed. The DEGs and critical module genes were intersected to obtain critical genes. Immune infiltration and competing endogenous RNA networks were also analyzed. Validation was conducted using quantitative real-time polymerase chain reaction. Hepatic injury was evident in DR rats. There were 2430 DEGs between DR and Sham and 261 DEGs between ER and DR. DEGs were mostly enriched in metabolic process for DR versus Sham, and immune and inflammatory processes for ER versus DR. 4 critical genes (Tff3, C1galt1, Cd48, and MGC105649) were obtained by screening. 5 immune cells were significantly different between DR and Sham, and 7 immune cells were significantly different between ER and DR in immunoassays. 3 critical genes, 75 miRNAs, 7 lncRNAs and 197 edges constituted the mRNA-miRNA-lncRNA linkages, which included C1galt1-rno-miR-330-5p-Pvt1, etc. This is the first attempt to perform a high-throughput analysis of gene expression profiles in DR-induced hepatic injury. It shows that immunity and inflammation-related RNAs and pathways play an important role in the progression of hepatic injury. It also provides insight into some important RNAs and regulatory targets related to disease.Study type: Original article. Does not apply.