Abstract
Male sterility (induced or natural) is a potential tool for commercial hybrid seed production in different crops. Despite numerous endeavors to understand the physiological, hereditary, and molecular cascade of events governing CMS in cotton, the exact biological process controlling sterility and fertility reconstruction remains obscure. During current study, RNA-Seq using Ion Torrent S5 platform is carried out to identify ‘molecular portraits’ in floral buds among the Cytoplasmic Genic Male Sterility (CGMS) line, its near-isogenic maintainer, and restorer lines. A total of 300, 438 and 455 genes were differentially expressed in CGMS, Maintainer, and Restorer lines respectively. The functional analysis using AgriGo revealed suppression in the pathways involved in biogenesis and metabolism of secondary metabolites which play an important role in pollen and anther maturation. Enrichment analysis showed dearth related to pollen and anther’s development in sterile line, including anomalous expression of genes and transcription factors that have a role in the development of the reproductive organ, abnormal cytoskeleton formation, defects in cell wall formation. The current study found aberrant expression of DYT1, AMS and cytochrome P450 genes involved in tapetum formation, pollen development, pollen exine and anther cuticle formation associated to male sterility as well as fertility restoration of CGMS. In the current study, more numbers of DEGs were found on Chromosome D05 and A05 as compared to other chromosomes. Expression pattern analysis of fourteen randomly selected genes using qRT-PCR showed high concurrence with gene expression profile of RNA-Seq analysis accompanied by a strong correlation of 0.82. The present study provides an important support for future studies in identifying interaction between cyto-nuclear molecular portraits, to accelerate functional genomics and molecular breeding related to cytoplasmic male sterility studies in cotton.
Highlights
Cotton, (Gossypium spp.) belonging to the Malvaceae family, that is an important cash crop because of being a natural source of fiber for various industrial use
The present study was resulted to identify the differentially expressed genes (DEGs) involved in cytoplasmic male sterility’ (CMS) pathways by comparing the Cytoplasmic Genic Male Sterility (CGMS), maintainer and restorer lines of cotton (Gossypium hirsutum L.)
The cDNA libraries for three lines i.e. JS178, JB178 and JR178 during sporogenous cells stage (SS) and microsporocyte stage (MS) stages were prepared separately, and reads were pooled for both stages of each line accounting 53.05, 61.70 and 67.96 million raw reads, respectively
Summary
Cotton, (Gossypium spp.) belonging to the Malvaceae family, that is an important cash crop because of being a natural source of fiber for various industrial use. Cotton is a major source of oil for human consumption and cottonseed meal provides important protein nutrients as animal feed [1] It provides more than 40% of world’s raw fiber for industrial use in more than 100 countries of temperate, tropical and subtropical regions, truly bestowed the name “white gold” [2]. Plant breeders of India and China [3] are able to increase yield of cotton from 10% to 20% by successfully acceptable limit of exploit heterosis using selective breeding [4]. Most of these heterosis are obtained through quite laborious, tedious, and costly process of artificial emasculation. This prompted scientists to use a novel technology called ‘male sterility’, as a tool for commercial hybrid seed industry, as it escape the need for hand emasculation in hybrid seed production [5]
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