Abstract
Tessaratoma papillosa is a major pest of Litchi chinensis and Dimocarpus longan. Adult and nymph secretions are not only harmful to plants but also to humans. At present, there are not a lot of research on T. papillosa, especially omics research. We used high-throughput sequencing technology to sequence the T. papillosa transcriptome and obtained 67,597 unigenes homologous to Halyomorpha halys (88.03%). Subsequently, RNA-SEQ and comparative analyses were performed on the 14 different developmental stages and tissues. A total of 462 unigenes related to growth and development, 1,851 unigenes related to digestion and detoxification, and 70 unigenes related to olfaction were obtained. Moreover, expression analysis showed that the T. papillosa major life activities genes are uniformly expressed across all developmental states. However, the adult midgut gene expression patterns were utterly different from that of the nymphs. Similarly, female fat body genes exhibited distinct expression patterns compared to that of males and nymphs. Thus, different developmental stages and physiological functions affect gene expression patterns. We also found that most of the differential genes were associated with cellular maintenance. This study will help understand the growth and development of litchi stink bugs, their choice of host plants, food digestion and detoxification, and their reproductive behavior. In addition, this result can provide reference information for some target genes in the process of control of T. papillosa.
Highlights
The selection of host plants by herbivorous insects relies on sensory signals, including vision, smell, taste, and touch
Insects take up some secondary plant metabolites together with the nutrients, and these metabolites eventually affect the normal physiological processes of insects (Pan et al, 2016; Yuan et al, 2020)
T. papillosa is a major pest of longan and lychee trees in Southeast Asia
Summary
The selection of host plants by herbivorous insects relies on sensory signals, including vision, smell, taste, and touch. The 1-dodecene is located on the egg surfaces laid on host plants Moths detect this compound through the OBP10 in their antennae and avoid the plants containing the eggs; this mechanism helps prevent cannibalism (Sun et al, 2012) at the same time, to counter the effects of the plant metabolites, insects have evolved various physiological and biochemical adaptation mechanisms. The intrinsic factors regulating this mechanism mainly depend on the abundance and expression patterns of the insects’ digestive and detoxification enzyme genes (Yang et al, 2001) These genes play a vital role in the growth and development of insects, and they are important target genes in pest control
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