Abstract
As one of the main phytohormones, abscisic acid (ABA) has been recently studied to regulate the fruit qualities during the post-harvest storage. However, the mechanism of the ABA affected the texture changes of post-harvest fruits remained unclear. In this study, the mature fruits of astringent persimmon ‘Gongcheng-shuishi’ with firm flesh had been treated with 300 mg/L ABA. Compared to the control group, the decreasing of fruit firmness as well as color development had been enhanced by exogenous ABA during post-harvest storage. A total of 300 differentially expressed genes (DEGs) had been isolated by means of Weighted Gene Co-Expression Network Analysis (WGCNA) and predicted to be associated with the changes of fruit firmness and color index. Among these DEGs, DkPG6, DkXTH13, DkBG11/12 showed highly correlated with cell wall metabolism, and DkPSY2 with the carotenoid accumulation. There were totally 12 differentially expressed transcript factors (DETFs) among these 300 DEGs. As transcript activator and repressor respectively, DkZAT4 (ZAT-LIKE TRANSCRIPTION FACTOR) and DkTCP4 (TCP TRANSCRIPTION FACTOR) could bind to and regulate the transcript activity of DkBG11 promoter significantly based on the results of dual luciferase assay and electrophoretic mobility shift assay. These results deepened the studying on the mechanism of ABA regulation on post-harvest fruit texture changes.
Published Version
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