Abstract
Prostaglandin-H-synthase-1 (PGHS-1), while constitutively expressed in most tissues, increases in abundance in human gestational membranes at term. This suggests that PGHS-1 may be up-regulated in preparation for labor, and thus might be a key determinant in timing labor onset. We conducted transient transfection experiments in amnion-derived AV3 cells utilizing pPGHS1CAT to identify substances that might regulate PGHS-1 expression in amnion. Transforming growth factor-β (1 ng/ml) and 15-deoxy-Δ 12,14 prostaglandin J 2 (1μM) significantly ( P < 0.05) (33% and 44% respectively) increased PGHS-1 promoter activity. The activity decreased significantly ( P < 0.05) in response to interleukin-1 (IL-1)β (1 ng/ml) (45%), tumor necrosis factor (TNF)-α (50 ng/ml) (34%), epidermal growth factor (10 ng/ml) (54%), phorbol myristate acetate (10 nM) (70%), IL-4 (10 ng/ml) (50%), IL-8 (100 ng/ml) (72%) and Activin A (25 ng/ml) (32%). Whether this degree of change in promoter activity leads to physiologically relevant alterations in the amounts of PGHS-1 present in cells remains to be determined.
Published Version
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