Abstract
Introduction Numerous molecular epidemiology studies have been performed about the frequency of Helicobacter pylori virulence genes in patients with H. pylori infection so far. This study was conducted to detect transcriptional profile by cDNA of H. pylori virulence genes in gastric biopsy samples of gastritis and gastric carcinoma patients. Materials and Methods In a case-control study, based on the prevalence of gastritis and gastric cancer in Sanandaj city during 2018 and 2019, 23 and 11 gastric antral biopsy samples with H. pylori infection were collected from gastritis and gastric carcinoma patients by the consecutive and available sampling method. Pathological characters, including tumor grades and tumor areas for gastric carcinoma biopsy samples prepared from gastric cancer areas, were determined by the pathologist. Total RNA of gastric antral biopsy samples was extracted, and their cDNA was synthesized by TaKaRa kit. H. pylori virulence genes' cDNA using specific primers and PCR was detected. This study's results were analyzed by SPSS version 25 and statics chi-square tests for determination of relationship and correlation between cDNAs of H. pylori transcriptional profile and clinical outcomes of H. pylori infection, including gastritis, gastric carcinoma, tumor grades, and tumor area. Results The positive statistical correlations were observed between transcripts of cagA, cagA-EPIYAC, cagE, and cagY genes and H. pylori infection clinical outcomes (P < 0.05). Conclusion Detection of the H. pylori virulence genes' cDNA in gastric biopsy samples can help provide the prognosis of clinical outcomes.
Highlights
Numerous molecular epidemiology studies have been performed about the frequency of Helicobacter pylori virulence genes in patients with H. pylori infection so far. is study was conducted to detect transcriptional profile by cDNA of H. pylori virulence genes in gastric biopsy samples of gastritis and gastric carcinoma patients
H. pylori chronic infection is followed by chronic mild gastritis with or without clinical manifestations, which progresses to gastric cancer in 1-2% of patients with H. pylori cagApositive genotypes infection [5]. e molecular epidemiology studies show a positive correlation between H. pylori virulence genes’ frequency and the clinical outcomes [6, 7]
50 gastritis and 30 gastric carcinoma biopsy samples were collected by the consecutive and available sampling method from patients referred to Tohid and Shaheed Ghazi hospitals in Sanandaj city for 18 months from September 2018 to March 2019. 23 (46%) and 11 (66.36%) patients had been infected with H. pylori in gastritis and gastric carcinoma patients, respectively
Summary
Numerous molecular epidemiology studies have been performed about the frequency of Helicobacter pylori virulence genes in patients with H. pylori infection so far. is study was conducted to detect transcriptional profile by cDNA of H. pylori virulence genes in gastric biopsy samples of gastritis and gastric carcinoma patients. Numerous molecular epidemiology studies have been performed about the frequency of Helicobacter pylori virulence genes in patients with H. pylori infection so far. Is study was conducted to detect transcriptional profile by cDNA of H. pylori virulence genes in gastric biopsy samples of gastritis and gastric carcinoma patients. In a casecontrol study, based on the prevalence of gastritis and gastric cancer in Sanandaj city during 2018 and 2019, 23 and 11 gastric antral biopsy samples with H. pylori infection were collected from gastritis and gastric carcinoma patients by the consecutive and available sampling method. H. pylori chronic infection is followed by chronic mild gastritis with or without clinical manifestations, which progresses to gastric cancer in 1-2% of patients with H. pylori cagApositive genotypes infection [5]. Some studies have concluded that the H. pylori persistence infection is due to the antigenic phase variations in H. pylori outer membrane proteins, including Hop, Bab, Sab, Oip, Dup, and Alp proteins [15,16,17,18,19,20].
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