Abstract
Leaf senescence is the last period of leaf growth and a dynamic procedure associated with its death. The adaptability of the plants to changing environments occurs thanks to leaf senescence. Hence, transcriptional profiling is important to figure out the exact mechanisms of inducing leaf senescence in a particular crop, such as rice. From this perspective, leaf samples of two different rice genotypes, the brown midrib leaf (bml) mutant and its wild type (WT) were sampled for transcriptional profiling to identify differentially-expressed genes (DEGs). We identified 2670 DEGs, among which 1657 genes were up- and 1013 genes were down-regulated. These DEGs were enriched in binding and catalytic activity, followed by the single organism process and metabolic process through gene ontology (GO), while the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the DEGs were related to the plant hormone signal transduction and photosynthetic pathway enrichment. The expression pattern and the clustering of DEGs revealed that the WRKY and NAC family, as well as zinc finger transcription factors, had greater effects on early-senescence of leaf compared to other transcription factors. These findings will help to elucidate the precise functional role of bml rice mutant in the early-leaf senescence.
Highlights
Leaf senescence is the ending period of leaf growth in the vegetative cycle, and acts as an indicator of physiological maturity of the harvest product
SeInnt.eJ.sMcoel.nScci.e201w9, 2a0s, xfFoOuR PnEdERtRoEVsIEtWart in the bottom leaves at the early-heading3 osft1a8ge in the bml mutant, whe2r.e1.inPhtehnoetyopitchCehrarlaectaervizeastiotnhen became yellow with progressive senescence (Figure 1A)
A total 16,510 genes was assigned in Gene Ontology (GO) and 1503 (9.10%) differentially-expressed genes (DEGs) annotated significantly in the GO database, among them 537 and 966 genes were regulated down- and up, respectively
Summary
Leaf senescence is the ending period of leaf growth in the vegetative cycle, and acts as an indicator of physiological maturity of the harvest product. Many reports have pointed out that it plays adaptive roles in plants in changing environments by fine tuning the regulatory genes [1,2] The majority of these genes are involved in (1) recycling and re-using the nutrients from senescent leaves for newly developed organs, and (2) enhancing tolerance against biotic or abiotic stresses [3,4,5]. The senescence process might be affected by internal as well as the external factors. The latter case is environmental conditions, e.g. soil moisture, light, temperature, nutrient content and pathogens [10,11]. Exploration of that mechanism will provide a means for controlling leaf senescence in crop plants [2]
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