Abstract
Fruit extract of neem (Azadirachta indica A. Juss.) induces defense response through enhanced activities of phenylalanine ammonia-lyase (PAL), tyrosine ammonia-lyase (TAL), polyphenol oxidase (PPO), peroxidase (POX) along with isoenzymes of PPO and POX in tomato. The increase in PAL, POX activity and induction of specific isoenzymes of acidic PPO, POX and basic POX by neem fruit extract were completely inhibited due to pre-treatment with actinomycin D or cycloheximide. Their activation was found to be time course-dependent. Synergistic enhancement of TAL activity by neem extract was observed when plants were pre-treated with cycloheximide, whereas similar observations were made for PPO activity by neem extract when plants were pre-treated with either actinomycin D or cycloheximide. These results suggest that the enhanced activities of defense enzymes and altered isoenzyme patterns of PPO and POX by neem extract was regulated at both transcriptional and translational levels which may contribute to the induction of defense responses in tomato.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
