Lignin metabolism involves Botrytis cinerea BcGs1- induced defense response in tomato

Chenyu Yang,Yingbo Liang,Dewen Qiu,Hongmei Zeng,Jingjing Yuan,Xiufen Yang

doi:10.1186/s12870-018-1319-0

Abstract

BackgroundBcGs1, a cell wall-degrading enzyme (CWDE), was originally derived from Botrytis cinerea. Our previous study revealed that BcGs1 could trigger defense responses and protect plants against various pathogens. We researched the defense response mechanism underlying this BcGs1 elicitation in tomato.ResultsWe revealed that the two domains were required for BcGs1’s full necrosis activity. According to analysis and quantitative real-time PCR of the up-regulated proteins and genes filtered by iTRAQ-based quantitative proteome approach, oxidative metabolism and phenylpropanoid metabolism were speculated to be involved in BcGs1-triggered defense response in tomato. Furthermore, experimental evidence showed that BcGs1 triggered reactive oxygen species (ROS) burst and increased the level of phenylalanine-ammonia lyase (PAL) and peroxidase (POD) enzyme activity, as well as lignin accumulation. Moreover, histochemical analysis revealed that infiltration of BcGs1 in tomato leaves exhibited cell wall thickening compared with untreated plants.ConclusionsThe results suggested that BcGs1 activated the basal defense response included lignin metabolism contributed to BcGs1-induced resistance to Botrytis. cinerea infection in tomato.

Highlights

BcGs1, a cell wall-degrading enzyme (CWDE), was originally derived from Botrytis cinerea
BcGs1-induced necrosis activity and resistance to Botrytis cinerea in tomato To investigate the necrosis activity in various host plants, we first obtained protein BcGs1 from the fermentation of Botrytis cinerea according to the method of Zhang, et al [23]
To analyze the appropriate induction time for disease resistance against Botrytis cinerea in tomato, fully mature 4-week-old tomato leaves were infiltrated with 0.25 μM BcGs1 solution at opposite sides of the central vein, and Tris-HCl buffer was used as control

Summary

Introduction

BcGs1, a cell wall-degrading enzyme (CWDE), was originally derived from Botrytis cinerea. To protect themselves against microbial colonization, plants have evolved a variety of defense mechanisms, including constitutive and inducible resistance strategies. In order to Start the relevant resistance system in plants a specific receptor to identify the pathogen is required, at the same time, the plant cell wall-derived molecules may be involved [1]. To overcome the barrier of the plant cell wall, phytopathogenic fungi secrete various CWDEs, such as cellulases, pectinase, hemicellulases, cutinase and protease. Most of these enzymes degrade cell wall components to get carbon sources for pathogen growth but can trigger multiple plant defense responses

Methods

Results

Discussion

Conclusion