Abstract
The ras proteins (Harvey, Kirsten and N-ras) are key regulators of signal transduction and a perturbation of their GDP/GTP cycle is frequently observed in tumors. In mammals, N- ras constitutes with unr ( u pstream of N - r as) a tightly linked tandem of ubiquitously expressed genes. Although unr and N- ras appear to be involved in distinct functions, this unusual genetic organization could be important for the regulation of N- ras expression. Specifically, transcription of unr could negatively regulate that of N- ras by transcriptional interference. To investigate this possibility, we have deleted the unr promoter by homologous recombination in murine embryonic stem cells. Analysis of tissues of heterozygous mice revealed an increase in N- ras mRNA accumulation ranging between 20 and 65%, in agreement with the suppression of a transcriptional interference.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
