Abstract
The transcription of the repetitive DNA sequences from salivary glands of Rhynchosciara angelae was investigated using RNA extracted from nuclei or cytoplasm prepared from these glands. For this purpose, we have used a new variation of methods carrying out and analysing hybridization experiments. It was found that both the highly and the intermediate repetitive DNA fractions have sequences complementary to nuclear RNA molecules. RNA sequences complementary to reiterated DNA could not be detected in the cytoplasmic RNA preparations, suggesting that these sequences are largely confined to the cell nucleus. Our data also indicate that the DNA sequences which undergo amplification simultaneously with the appearance of the giant DNA puffs are transcribed into RNA sequences which are not transported to the cell cytoplasm.
Published Version
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