Transcription of non-repeated DNA in Neurospora crassa

Abstract

Repeated and non-repeated DNAs of Neurospora crassa mycelial cells were fractionated by hydroxyapatite chromatography and characterized separately for reassociation kinetics and thermal stability profiles of reassociated DNAs. Approx. 90 % of N. crassa DNA sequences reassociated like single-copy DNA. About 35 % of these unique sequences were found to be transcribed in mid-log phase of mycelial growth when measured by DNA · RNA hybridization at a RNA C 0t of about 90 000.