Abstract

The transcription of non-repeated DNA sequences of four developmental cell types of the eukaryotic fungus Neurospora crassa was measured quantitatively by DNA:RNA hybridization using hydroxyapatite chromatography. DNA transcription was 34% (68% of the genome) at midlog phase of mycelial growth and 15% at conidial phase. Conidial sprouts and unbranched mycelial cells showed markedly increased transcription, 25% and 27% respectively. DNAs from mycelia and conidia did not show any difference in nucleotide base composition and sequence homologies. It was concluded that approximately 30% of N. crassa genome consist of sequences which are involved either in cell differentiation and development.

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