Abstract
The aim of this study was to determine if biocytin, a low molecular analog of biotin, could reliably label details of corticospinal fibers at the lumbar level in rats. Biocytin (5%) was injected extracellularly by pressure into the unilateral hindlimb area of the motor cortex. Frozen sections of the injection sites and the lumbar segments were incubated with avidin-horseradish peroxidase (HRP). Terminal labeling of corticospinal fibers at the lumbar segments could be observed within 2–3 days of the injection. These results indicate that anterograde tracing with biocytin can be applied to label axons in a long tract such as the corticospinal tract in rats. Biocytin stain provided a view of a higher level of detail than wheat germ agglutinin (WGA)-HRP.
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