Abstract
Trace minerals participate in reproductive processes and are crucial for oocyte maturation. The objective of the present study was to investigate the effect of combined supplementation with copper (Cu), manganese (Mn), selenium (Se) and zinc (Zn) during bovine in vitro maturation (IVM) on subsequent embryo development and quality. The IVM medium was supplemented as follows: a) Control (no mineral supplementation); b) MScz (6ng/mL Mn + 100ng/mL Se + 200ng/mL Cu + 400ng/mL Zn); c) MScZ (6ng/mL Mn + 100ng/mL Se + 200ng/mL Cu + 1200ng/mL Zn); d) MSCz (6ng/mL Mn + 100ng/mL Se + 600ng/mL Cu + 400ng/mL Zn). Supplementation with MScz and MSCz produced more blastocysts compared with the control. Total blastocyst cell number was higher when minerals were added at any combination. Day-8 blastocysts derived from oocytes treated with minerals had lower intracellular reactive oxygen species concentration and lipid content than the control. In conclusion, combined supplementation with Cu, Mn, Se and Zn during bovine oocyte IVM increased in vitro production performance, improving embryo developmental ability and quality.
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