Trace level enrichment and clean-up of β-lactam antibiotic residues in edible chicken tissues and feathers by solid phase extraction for quantitative determination utilizing liquid chromatography

Abstract

ABSTRACT. A simple analytical method for the extraction and determination of three β-lactam antibiotics in chicken tissues and feather samples was developed and validated using solid phase extraction (SPE) and liquid chromatography-ultraviolet, LC-UV, detection. The analytes were extracted with strong anion exchange (SAX) cartridges for the extract clean-up and enrichment before introduction into the LC system. The validation was carried out based on the recommendations from Commission Decision 2002/657/EC. Under optimised conditions, the proposed method offers correlation coefficients of r2 ≥ 0.9991 for all the studied analytes in both matrices. Percent recovery results were found to vary between 79.7-101.8% and 94.2-101.6% for chicken feathers and tissues, respectively, with the relative standard deviation, RSD < 8.4%. The limits of detection (LOD) were in the range of 0.1-0.13 µg kg-1 and 0.1-0.15 µg kg-1 while the limits of quantification (LOQ) were in the range of 0.3-0.38 µg kg-1 and 0.3-4.5 µg kg-1 in chicken feathers and tissues, respectively. The proposed method offers good sensitivity and selectivity and all the results demonstrating that the method would be widely used for determination of β-lactam antibiotics and other trace level antibiotics residues having similar physical and chemical properties in the matrices of varying origins.
 
 KEY WORDS: Chicken tissues, Chicken feathers, β-Lactams, Solid phase extraction, LC-UV
 
 Bull. Chem. Soc. Ethiop. 2022, 36(3), 503-519. 
 DOI: https://dx.doi.org/10.4314/bcse.v36i3.2