Abstract
Capillary zone electrophoresis was developed for the simultaneous determination of haloperidol (HP) and its chiral metabolites [(+)- and (−)- reduced haloperidol, (+)- and (−)-RHP] in human plasma. The method involved the presence of an internal standard and liquid–liquid extraction from plasma. After concentration, the residue from the organic extract was dissolved in aqueous acid for capillary electrophoretic analysis. The background electrolyte was Tris–phosphate buffer with dimethyl-β-cyclodextrin and PEG 6000. In spiked plasma the quantitative ranges were 40–400 n M for HP and 50–500 n M for (+)-RHP or (−)-RHP. The intra-day and inter-day relative standard deviations ( n=3) were all <20% for each substance. The detection limits were found to be 15 ng/ml for HP and 30 ng/ml for both enantiomers of RHP (S/N=3, injection 20 s). All recoveries were >70%. We investigated the in vivo metabolism of HP in Chinese schizophrenia patients. The results show that (−)-RHP seems to be the only chiral metabolite from these two HP-dosed patients.
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