Abstract
A general method for the trace analysis of benzodiazepine drugs and their major metabolites at single dose therapeutic levels in 0.2 ml blood samples is described. The method involves solvent extraction of blood with toluene, isolation of the analytes using deactivated Amberlite XAD-7 porous polymer beads, and analysis of the cleaned-up extracts by capillary column gas chromatography with electron-capture detection. The clean-up technique eliminates lipids and other interfering material, enabling routine analysis of blood extracts to be carried out with no significant deterioration in column or detector performance over a period of many months. The use of fused-silica capillary columns coated with SE-52 and the correct choice of chromatographic conditions permits underivatised benzodiazepines of widely differing volatilities and polarites to be analysed. Data for 26 benzodiazepines and metabolites are presented.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
