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Abstract
BackgroundTargeting protein for Xenopus kinesin-like protein 2 (TPX2) is a microtubule-associated protein required for microtubule formation in human cells. Several studies have demonstrated that TPX2 is overexpressed in multiple tumor types and promotes tumor growth and metastasis. However, there have been few reports regarding its role in gastric cancer. In this study, we evaluated TPX2 expression and investigated its correlations with gastric cancer clinicopathological features and prognosis.MethodsTumor samples were obtained from 290 patients with gastric adenocarcinoma who had undergone gastrectomy. The expression of TPX2 protein was examined using immunohistochemical staining. TPX2 messenger RNA (mRNA) levels were evaluated using real-time quantitative reverse transcription PCR in 19 of the gastric cancer tumors and adjacent normal tissues.ResultsThe mRNA levels of TPX2 were significantly higher in gastric cancer tissues than in matched adjacent normal tissues (p = 0.004). In the immunohistochemical analysis, TPX2 overexpression was found in 123 (42.4%) of 290 patients. High TPX2 expression was positively associated with age, type of histology, depth of tumor, lymph node metastasis, stage, and remote metastasis or recurrence. High TPX2 expression was significantly associated with poorer disease-specific survival (p = 0.004) and relapse-free interval (p = 0.013).ConclusionsOur results indicated that high TPX2 expression was associated with tumor progression and poor survival in gastric cancer.
Highlights
Targeting protein for Xenopus kinesin-like protein 2 (TPX2) is a microtubule-associated protein required for microtubule formation in human cells
The median TPX2 messenger RNA (mRNA) level was significantly higher in gastric cancer tissues than in matched adjacent normal tissues (p = 0.004; Fig. 1)
Immunohistochemistry for TPX2 expression Expression of the TPX2 protein was assessed by immunohistochemical staining of gastric cancer tissue samples from 290 patients
Summary
Targeting protein for Xenopus kinesin-like protein 2 (TPX2) is a microtubule-associated protein required for microtubule formation in human cells. We evaluated TPX2 expression and investigated its correlations with gastric cancer clinicopathological features and prognosis. Levels of targeting protein for Xenopus kinesin-like protein 2 (TPX2) were found to correlate with chromosomal instability [7]. Its expression is tightly regulated by the cell cycle, and this protein is detected during the G1-S stage and disappears after the completion of mitosis. As a critical regulator of mitosis, TPX2 cooperates with Aurora-A kinase and Eg5 kinesin to control microtubule assembly and spindle stability. TPX2 is required for the formation of normal bipolar spindles and chromosome segregation [9, 10]
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