Toxicological study of the new gel containing oak bark extract and aloe extract under long-term use

D Zhurenko,N Tsubanova

doi:10.24959/ubphj.19.237

Abstract

Topicality. The significant prevalence of destructive-inflammatory periodontal diseases and the imperfection of existing medicines determine the feasibility to develope new topical dosage forms with polymodal pharmacological activity and low toxicity. Scientists of NUPh developed a new gel, containing an oak bark extract and aloe extract with strong parodontoprotective effect.Aim. To study the toxicity of a new gel containing an oak bark extract and aloe extract under the long-term use. This new gel is developed by the scientists of NUPh, under the direction of prof. N. V. Khokhlenkova, and is intended for treatment of oral cavity inflammatory diseases. In previous studies a pronounced membran-protective, antimicrobial, anti-inflammatory activity and parodont-protective activity were established for the new gel.Materials and methods. Prior to the experiment, randomized samples were applied for the animal population taken in experimental groups and fixed averaged indicators of the initial state of animals organism. A new gel was applied on the skin.Results and discussion. Animals that received the new gel did not differ from the animals of the control groups in appearance, behavior and attitude to food. During the experiment, there were no signs of inhibition of activity and development of aggressiveness in animals of comparable groups. The results of the study of weight ratios of both sex rat organs indicate the absence of test gel toxic effects in doses of 0.5 and 5 ml. The new gel does does’t reveal hepatotoxic effect. The results of lipid metabolism indices shows that there is no toxic effect in the studied doses.Conclusions. It was established that a new gel, containing oak bark extract and aloe extract, does not exert a toxic effect on the functions of vital organs and systems of the organism of experimental animals at the conditions of prolonged application (1 month) at doses of 0.5 ml and 5 ml. Along with the previously established parodontprotective, antimicrobial and other activities, the absence of toxic effects of a new gel under the conditions of long-term use is the basis for in-depth preclinical studies to create a new drug with parodontprotective effect.

Highlights

The most important characteristic of any drug, along with the experimentally proven high efficiency, is low toxicity
Animals that received the new gel did not differ from the animals of the control groups in appearance, behavior and attitude to food
It was established that a new gel, containing oak bark extract and aloe extract, does not exert a toxic effect on the functions of vital organs and systems of the organism of experimental animals at the conditions of prolonged application (1 month) at doses of 0.5 ml and 5 ml

Summary

Introduction

The most important characteristic of any drug, along with the experimentally proven high efficiency, is low toxicity. Features of the toxicological profile are the main obstacles to the introduction of new drugs and restricts medical use of already registered drugs in the context of long-term use [1,2,3]. In this regard study of the toxicity of new drug in terms of long-term use is the mandatory condition of preclinical studies [4,5,6]. Aim of the work was to study the toxicity of a new gel which contains an extract of oak bark and extract of aloe in terms of long-term usage. The aim is to study the toxicity of a new gel containing an oak bark extract and aloe extract in long-term use

Methods

Results

Conclusion