Abstract
The median lethal concentrations (LC 50s) of tri- n-butyl-tin oxide (TBTO) to juvenile chinook salmon, Oncorhynchus tshawytscha, adapted to seawater were determined in a static renewal bioassay. LC 50s were 54, 20, and 1.5 μg TBTO/l after exposures for 6, 12, and 96 h, respectively. LC 50s decreased logarithmically with time for exposures between 12 and 96 h. Also determined were the average tri- n-butyl-tin (TBT) concentrations in liver, brain, and muscle tissues of salmon that died during the bioassay: 7.0, 3.5, and 0.52 μg TBT/g wet weight tissue, respectively. TBT concentrations in liver, brain, and muscle tissues of salmon that survived until day 4 of the bioassay were 4300, 1300, and 200 times exposure concentrations, respectively. Our results implicate TBT exposure as the cause of death of chinook salmon exposed to TBT-treated marine net pens at one aquaculture facility.
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