Abstract
Abstract As a basis for embryo cryostorage research, information on relative toxicities of cryoprotectants is of key importance. Both morulae and tail-bud-stage embryos of red drum (Sciaenops ocellatus) were exposed to cryoprotectant solutions for 20 min at room temperature. Artificial seawater (ASW) at 30–32‰ was used as a medium. Embryos were exposed to solutions in a one-step addition. Toxicity was evaluated in terms of percentage hatch and 40-h survival. Maximum concentrations tolerated by morulae without significant reduction in hatch and survival were 1 M glycerol, 2 M dimethyl sulfoxide (DMSO), 2 M methanol, 0.5 M sucrose, and 95‰ ASW. Ethylene glycol was toxic to morulae even at 0.25 M. Tail-bud embryos were more resistant, with maximum innocuous concentrations being 1 M glycerol, 2 M DMSO, 2 M ethylene glycol, 2 M methanol, 0.5 M sucrose, and 95‰ ASW. Serial addition and dilution of 95‰ ASW offered no advantage over a direct plunge and rinse. Maximum harmless concentrations may not afford adequat...
Published Version
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