Toxicity of Insecticides to Larvae of Banded Cucumber Beetles (1982)

Abstract

Abstract The soil bioassay used for determining toxicity of insecticides to western, northern, and Mexican corn rootworm larvae was followed explicitly in this study. The soil, classified as a Brookings silty clay loam, had a pH of 7.1 and was 4.2% organic matter. BCB larvae were reared in the laboratory on corn seedlings. For each bioassay, six soil/ insecticide concentrations that ranged from 0 ppm to a level that induced 100% larval mortality were mixed using technical grade toxins; each mixture was divided into 4 to 6 samples. Ten early 3rd-stage BCB larvae, ca. 9-10 days old and weighing 3 to 5 mg, were placed in each sample and incubated at 25°C for 48 h. Dose mortality responses were calculated using a SAS probit analysis.