Toxicity Effect of Cu2+ Contaminated Kitchen and Vegetable Wastes on Oxidative Stress Response of Black Soldier Fly Larvae, Hermetia Illucens

Eman Alaaeldin Abdelfattah,Aya M Aboelhassan,Hamid Ashry,Habiba Mohamed Ibrahim,Dina H Abd El-Monem,Mohammed Y Hussein,Dyaa Bassiony,Mohamed A Fawzy,Aya H Mahmoud,Abdallah Nagah,Nada Y Ibrahim

doi:10.32861/jbr.81.7.25

Abstract

Environmental pollution sources including waste or metal accumulation, industrial and agricultural activities can be dangerous. Also, contaminated organic waste (COW) with metals especially, copper ions (Cu2+), can cause toxicity to various ecosystem components, enhance the production of reactive oxygen species (ROS) and consequently cause oxidative stress. The biochemical effect of the COW was monitored by assessing the oxidative stress parameters (OSP) using hydrogen peroxides (H2O2), protein carbonyls (PC), lipid peroxides (LP), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), 2,2 diphenyl-1-picrylhydrazyl (DPPH), total antioxidant ability (TAA); β-esterase (β-EST); and total amount of protein (TAP) levels on the organic waste (kitchen and vegetable wastes); Hermetia illucens larvae; and larval excreta collected from 7-day post-treated kitchen and vegetables wastes with (1:10; g:mL) distilled water (DW) or 100 mg/mL Cu2+. The OSP levels were significantly higher in the experimental samples from Cu2+ groups than in the control one. Besides that, the OSP levels of H. illucens larvae feed on vegetable waste was significantly higher than those feed on kitchen waste except for β-EST, PC, and TPA. The best, cheap and easy parameters of antioxidants to ensure the entomoremediation ability are total antioxidant capacity. Possible impacts of accumulated and Cu2+ contaminated organic waste on H. illucens larvae were discussed. Also, the ability of insects to produce more antioxidants than input or output sources was approved. The potential use of the OSP as a bioindicator method of the bioremediation ability of H. illucens was proposed.

Highlights

The effects of various chemical or environmental pollutants on the insects’ physiology and biochemistry have been investigated in various studies [1-9]
We want to answer some questions; can we manage organic waste contaminated with metals using BSF larva (BSFL)? What is the validity of using oxidative stress parameters as an indicator of bioremediation ability? Are BSFL having the ability to produce more antioxidants especially within polluted zones? the main aim of the present work is the measurement of oxidative stress and detoxification levels in the various types of organic waste, BSFL, and insect excreta
The levels of H2O2, protein carbonyls (PC) and lipid peroxides (LP) were generally lower in control than Cu2+-treated KW or vegetable waste (VW) waste samples (Table 1 and Fig. 1)

Summary

Introduction

The effects of various chemical or environmental pollutants on the insects’ physiology and biochemistry have been investigated in various studies [1-9]. Environmental pollution sources including waste accumulation, industrial and agricultural activities have a direct effect on the amounts of cupper elevation than normal levels. Excess of copper ions (Cu2+) can cause toxicity to various tissues [10], enhance the production of reactive oxygen species (ROS) and cause oxidative stress in living organisms, such as insects [5, 11-14]. Cu2+ are crucial for cellular biochemistry and physiology, as it is used as a cofactor of various enzymes, and factors of cellular processes such as oxidative stress defense, ion homeostasis, cellular respiration, neural transmission, tissue maturation, or iron metabolism [15, 16]. The main function mechanism of Cu2+ in living organisms is linked with its redox chemistry [3, 5, 18]

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