Abstract
In order to evaluate the effect of anaerobic digestion intermediates and antibiotics in pharmaceutical wastewaters on anaerobic digestion process, their acute toxicities were tested using the 15min median inhibitory concentration (IC50) at pH 7.00±0.05. The results showed that the IC50 of ethanol, acetate, propionate and butyrate were 19.40, 20.71, 10.47 and 12.17gL−1 respectively, which suggested the toxicity descended in the order of propionate, butyrate, ethanol and acetate. The IC50 of aureomycin, polymyxin and chloromycetin were 12.06, 6.24 and 429.90mgL−1 respectively, which indicated the toxicity descended in the order of polymyxin, aureomycin and chloromycetin. Using equitoxic ratio mixing method, the joint toxicities of five groups referred by A (four anaerobic digestion intermediates), B (four anaerobic digestion intermediates and aureomycin), C (four anaerobic digestion intermediates and polymyxin), D (four anaerobic digestion intermediates and chloromycetin) and E (four anaerobic digestion intermediates, aureomycin, polymyxin and chloromycetin) were investigated respectively. Their interactions were additive (A), synergistic (B), additive (C), synergistic (D) and synergistic (E). The investigation would lay a basis for the optimization of anaerobic biotechnology for pharmaceutical wastewater treatment.
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