Toxicity and trophic transfer of polyethylene microplastics from Poecilia reticulata to Danio rerio

Abstract

The potential transfer of microplastics (MPs) between vertebrates belonging to the same taxonomic group, and the impact of such a transfer on higher trophic levels remains little explored. An experimental food chain with two fish species was installed to test the hypothesis that polyethylene MPs (PE MPs) can accumulate in animals and cause behavioral, mutagenic and cytotoxic changes at upper trophic levels. Poecilia reticulata fry were exposed to MPs for 48 h and, subsequently, offered (as food) to Danio rerio adults for 10 days to simulate an upper level food chain. PE MPs quantification in fry and in different Danio rerio tissues evidenced their accumulation at the two assessed trophic levels. This finding suggested their absorption, adherence and translocation from one organism to another. The accumulation seen in D. rerio directly exposed to MPs was associated with behavioral disorders at upper trophic level. These animals presented behavior suggestive of anti-predatory response deficit when they were confronted with a potential aquatic predator (Geophagus brasiliensis). This finding was inferred through lower school cohesion, shallower school depth and shorter distance from the potential predator. In addition, animals exposed to MPs recorded higher nuclear abnormality rates and changes in the size and shape of erythrocytes and in their nuclei; this outcome has suggested mutagenic and cytotoxic effects, respectively. Based on the current results, MPs are transferred through a food chain that only involves two vertebrates. MPs enter the vertebrates' organs, change their behavior and induce mutagenic and cytotoxic processes in animals, which can cause significant ecological consequences in freshwater ecosystems.