Toxicity and biochemical action of the antibiotic fungicide tetramycin on Colletotrichum scovillei

Abstract

Tetramycin, a novel polyene macrolide antibiotic, has strong activity against a broad spectrum of fungi and may have potential uses in future agricultural applications. Thus, the antifungal activity and biochemical action of tetramycin on Colletotrichum scovillei were investigated in this study. The experimental results indicated that tetramycin had strong inhibitory activity against the mycelial growth, spore germination and germ tube elongation of C. scovillei. The baseline sensitivity curves were unimodal, with mean EC50 values of 1.98 ± 0.078 μg/mL and 0.003 ± 0.005 μg/mL for mycelial growth and spore germination inhibition, respectively. Tetramycin also inhibited the germination of spores and formation of appressoria. After tetramycin treatment, the edge of the mycelial diaphragm showed protuberances, with decreased offshoots at the top. Additionally, disruption of the membrane was detected through an increase in membrane permeability, leakage of sugars and a reduction in the ergosterol content. Tetramycin effectively controlled C. scovillei on detached pepper fruits. These results will contribute to our evaluation of the potential of tetramycin for successful management of pepper anthracnose and to our understanding of the possible biochemical action of tetramycin against C. scovillei.