Toxicant interactions with food algae: A missing link between laboratory and field effects?

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Algae fed to invertebrate subjects of chronic toxicity testing are cultured without exposure to test substances. This approach may reduce the ability of bioassays to predict field effects because it assumes that bioconcentration is the only important uptake route, and that an interaction between toxicant and algae does not occur or is not relevant to the effect of the toxicant on test animals. The research presented in this paper focuses on the effects of a bleached kraft mill effluent (BKME) on algae used as food for test animals and the possible consequences of this exposure to bioassay results. The experiment consisted of exposing cultures of a pennate diatom, Navicula, to a range (0–7%) of BKME concentrations for 15 days. Final biomass (measured as chlorophyll a and ash free dry mass) was significantly greater in cultures exposed to 5% and 7% BKME. The carbon-to-nitrogen ratio was significantly higher in diatom cultures exposed to 7% BKME, and total lipid content ranged from 11.7% in the control to 15.8% in the 7% treatment. BKME exposure also increased bacterial content and altered the elemental composition (particularly strontium, barium, iron, and cobalt) of Navicula relative to control cultures. Because changes in food abundance and food quality (e.g., dietary lipids, carbohydrates, proteins) are known to modify toxicity and because contaminant uptake can occur through ingestion, exposing algal food supplies to toxicants would allow chronic bioassays to better simulate field conditions. This approach would be of value in situations where bioassays are intended to predict field effects rather than to compare the toxic potential of effluent samples. Although culturing food algae under exposure to contaminants poses methodological challenges, this approach may serve to enhance the predictive ability of chronic bioassays. © 2001 John Wiley & Sons, Inc. Environ Toxicol 16: 31–42, 2001