Abstract
Causes of variation in numbers of females on a cultivar are examined. These are such that an accurate assessment of resistance in the field is not possible. The method used for a laboratory assay is as follows. Seed is selected for uniformity of size and pre‐germinated before sowing, when the seminal roots are about 1 cm long, in tubes of sandy loam. Tubes are opaque (2.5 cm internal diameter by 13 cm long) and are set on a base of potting compost. Seedlings are inoculated immediately with the required number of larvae in 1 mi water ‐ for oats 50 larvae, wheat 75 larvae and barley 100 larvae. They are inoculated at the same density at 3‐daily intervals on four further occasions. The aim is to produce 50 females on the most susceptible cultivar; densities have been determined from intolerant cultivars. Plants are grown under 10‐h day‐length at 15oC and are harvested 2 months after the last inoculation, at which time they are assessed and the valuable plants can be repotted and grown on for seed production.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
