Abstract
The separation and purification of chlorogenic acid (CA) from honeysuckle is a challenging yet significant task in the food industry. Traditional methods, such as precipitation and chromatography methods, often suffer from secondary pollution or low efficiency. This paper proposes a green and efficient adsorption method for separating CA from honeysuckle using melamine–formaldehyde aerogel (MFa) nanomaterial. The synthesis of MFa adsorbent with developed mesoporous material is crucial for achieving high efficiency. The optimal conditions for CA adsorption were determined to be an MFa dose of 0.03 g, a pH value of 3.72, and an adsorption temperature of 18.89 ℃. Under these conditions, a CA adsorption capacity of 151.84 mg/g and an adsorption ratio of 69.58 % were achieved, demonstrating the superior performance of the MFa method. Most notably, the purity of CA improved significantly from 10.50 % to 60.50 %, a marked enhancement improvement over traditional sorption resins. Additionally, the superior antioxidant effect of CA treated with the MFa method was confirmed at both the chemical assay and cytological levels. Affinity analysis and MD simulations revealed that CA adsorption is primarily driven by electrostatic interaction. This study not only addresses current limitations of honeysuckle but also broadens the application pontential of aerogel in food and medicine industries.
Published Version
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