Total Phenolic Compound and Antimicrobial Activity of Spirogyra spp.

Abstract

Spirogyra spp. is widely distributed in freshwater habitats and is included in the category of natural resources whose significance and advantages have been researched. In this study, 6 samples of Spirogyra spp. were collected: Spirogyra ellipsospora Transeua from Bueng Kan province, Spirogyra sp.1 from Sakon Nakhon Province and S. neglecta (Hassall) Kützing, S. ellipsospora Transeua, Spirogyra sp.2, and Spirogyra sp.3 from Nakhon Phanom province. The highest value of total phenolic compound was measured at 18.53±0.23 mg GAE/mg in Spirogyra sp. 2. The evaluation of antimicrobial activity of algal extracts against fungal Candida sp. and bacteria Staphylococcus aureus and Escherichia coli was performed by using the disk diffusion method along with determination of minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and minimum bacterial concentration (MBC). The result of disk diffusion and MIC determination showed the efficiency of all extract samples against all tested strains at a concentration of 10 mg/mL. The most effective algal extract against Candida sp. showed with the MFC’s ranged from 10 to 20 mg/mL, whereas MBC of all extracts destroyed the two tested bacterial strains ranged from 10 to 20 mg/mL within 24 hours and up to 30 mg/mL after 48 hours. The results of this study support that Spirogyra spp. contains bioactive compounds that are the basis for further application in cosmeceuticals.