Toros Göknarı (Abies cilicica (Antoine & Kotschy) Carrière) ’ndan Elde Edilen Reçinenin In vitro Antioksidan ve Sitotoksik Aktivitelerinin Belirlenmesi

Abstract

In this study, the antioxidant and cytotoxic activities of resin obtained from the Cilician Fir plant were evaluated. According to data, the in vitro antioxidant activity of the resin has changed depending on the radical scavenging activity method. This resin has antioxidant activity according to 2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay. The in vitro cytotoxic activity of the resin was investigated against a panel of human cancer cells (MDA-MB-231, Hep G2, PC-3, U-87, MCF-7, HT-29) with the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay for 48 h. Normal human lung fibroblast cells (WI-38) were used as healthy cells. The results indicated that the in vitro cytotoxic activity of the resin depends on the cell line type and concentration of the resin. According to the IC50 values, the resin has the most cytotoxic activity on endometrial adenocarcinoma cancer cells (IC50=8.94 ± 0.03 µg mL-1) compared to other cancer cells. The results also indicated that Ishikawa endometrial adenocarcinoma cells, which have Selectivity Index (SI) value >2, have the most sensitivity against the resin. This study provides the first evidence that the resin inhibits the different cancer cells' growth.