Abstract
A simple tabletop model of a versatile counter-current chromatographic system is introduced. The apparatus compactly holds a long coiled column around a drum-shaped holder. The acceleration produced by the synchronous planetary motion of the holder enables stable retention of the stationary phase in each helical turn of the coil while the mobile phase is continuously eluted through the column. The capability of the method was demonstrated on separations of DNP amino acids and oligopeptides using typical two-phase solvent systems. The present method will provide a universal application of solvent systems including aqueous-aqueous polymer phase systems used for partition of cell particles and macromolecules.
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