Abstract
Potential capability of the apparatus in performing countercurrent chromatography has been examined with three different types of coiled columns, all coaxially mounted around the holder. In single-layer coils, typical solvent systems display characteristic hydrodynamic distribution which ensures a stable retention of the stationary phase against heavy sample loading in preparative separations. The cross-axis synchronous flow-through coil planet centrifuge produces a novel pattern of the centrifugal force field which strongly suggests a high performance in countercurrent chromatography. Various two-phase solvent systems were examined using both the head and the tail phases as the mobile phase. Hydrodynamic motion and distribution of the two solvent phases in a rotating column was directly observed and photographed under stroboscopic illumination. The geometry of the spiral column creates a radial gradient in the centrifugal force field which favors the lower phase moving outward or the upper phase moving inward through the spiral column.
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